NIDA IRP - Genetic Engineering and Viral Vector Core

Genetic Engineering and Viral Vector Core

Christopher Richie, Ph.D.

Christopher Richie, Ph.D.

A NIDA IRP Core Facility

Core Manager

Christopher Richie, Ph.D.

Contact

Location:
Biomedical Research Center
251 Bayview Blvd
Suite 200
Baltimore, MD 21224

Phone: 443-740-2568

Email: gevvc@nida.nih.gov

About the Core

Mission: The Genetic Engineering and Viral Vector Core (GEVVC) provides support to NIDA-IRP researchers by facilitating studies of brain function under physiological and pathological conditions through developing and producing genetic tools capable of modulating and monitoring the molecules, cells and circuits in the nervous system.

Services:
The NIDA GEVVC provides services in three main categories: 1) the custom design and/or production of viral vectors/nanoparticles, 2) in vitro molecular and cell biology services, 3) consultation and training.

Icon - Recombinant Plasmids
Recombinant Plasmids

  • Construction, preparation, sequencing, and curation
  • Functional testing by transfection into cultured cells
  • Ectopic expression and purification of proteins (bacteria/mammalian cell culture)

Icon - Adeno-associated Virus

Adeno-associated Virus (AAV) Technology

  • Viral genome design and construction
  • Vector packaging (serotypes AAV1, AAV2, rAAV2-retro, AAV6, AAV8, AAV9, AAV-PHP.eB)
  • Functional testing by transduction into rat primary cortical neurons.

Icons - Lentivirus

Lentivirus (LV) Technology

  • Viral genome design and construction
  • Vector packaging (VSV-G pseudotype)
  • Functional testing by transduction into rat primary cortical neurons.
  • Transduction and selection to create stably-transduced cell lines

Icon - Genomic Editing

Genome Editing

  • Consultation and design of reagents for targeted mutagenesis in rodent models
  • Development of genotyping assays
  • Quantification of transgene copy number
  • BAC recombineering

Icon - Training
Training