AD/ADRD Single Cell Proteomics Think Tank Meeting

Purpose and Background

The advancements of single cell proteomics (sc-proteomics) technologies have now allowed investigators to directly describe the phenotypes of cells without the need to infer proteins from cellular mRNA levels. In this one-day think tank meeting, the speakers will present the state of the science on sc-proteomics and explore the potential opportunity to use this type of technology in AD/ADRD research.

This workshop will cover the following areas:

• High throughput single cell chemical and proteomic analyses
• Novel sample preparation and data acquisition for single cell analyses.
• Applications of using scProteomic analyses to study human brains.

Location

This meeting was held virtually on Zoom.

Registration Information

Registration is closed.

Agenda

Note: All meeting times are listed in Eastern Time.

11:00 a.m. Welcome and Introductions, Dr. Austin Yang, National Institute on Aging

11:10 a.m. Dr. Eliezer Masliah, National Institute on Aging

11:15 a.m. Dr. Jonathan Sweedler, University of Illinois: The high throughput chemical characterization of the cells in the brain

11:45 a.m. Dr. Hui Zhang, Johns Hopkins University: Data-Independent Acquisition Approach for Proteomic and PTM Analyses at Single-Cell Level

12:15 p.m. Dr. Ryan Kelly, Brigham Young University: Single cell proteomics: recent advances and promising new directions

12:45 p.m. Break

1:00 p.m. Dr. John Yates, Scripps Research Institute: Proteome Analysis using Combined Single Neuron Patch-Clamp / Mass Spectrometry (PatchC-MS)

1:30 p.m. Dr. Junmin Peng, St Jude Children’s Research Hospital: Exploring Single Cell Type Proteomics in Alzheimer's Disease

2:00 p.m. Lunch

2:30 p.m. Dr. Peter Nemes, University of Maryland: Single-neuron mass spectrometry proteomics in the mammalian brain

3:00 p.m. Dr. Nikolai Slavov, Northeastern University: Transformative opportunities for single cell proteomics

3:30 p.m. Discussion

4:30 p.m. Adjourn

Contact Information

Please contact Dr. Elizabeth Newman and Dr. Austin Yang for questions about this workshop.

DN